Mazene Hochane

Researcher, LACDR

I am a researcher at Leiden Academic Center for Drug Research (LACDR) in the group of Prof Bob van de Water, with a multidisciplinary background in developmental biology, stem cell systems, and cancer research besides a background in Pharmaceutical Sciences.

I completed my PhD in France, where I studied the molecular mechanisms underlying chronic kidney disease of glomerular origin. I also investigated the toxic effects of environmental contaminants on the biology of adult stem cells including their carcinogenic potential in postdoctoral residency in France.

I later moved to the Netherlands, holding postdoctoral positions at the Leiden Institute of Physics, where I studied early mammalian development, including gastrulation and kidney organogenesis using single-cell technique and organoid culture. At the LACDR, my main focus was on human embryonic stem cells-based disease modelling through reporter generation by CRISPR/Cas9, and protocol optimization for high-throughput imaging.

My current research centres on how cellular identity and plasticity contribute to epithelial pathologies, with a focus on pancreatic cancer. I use single-cell transcriptomics, organoid models, and toxicity screening approaches to explore the molecular dynamics of early tumorigenesis.

Presentation: Functional analysis of tumorigenic effect of environmental exposures using iPSC-based organoid model of the pancreas

Pancreatic cancer is one of the most lethal forms of cancer with 5-year global survival less than 5%. Pancreatic ductal adenocarcinomas (PDAC) account for about 90% of pancreatic cancer cases. Importantly, the prevalence of PDAC in Europe varies across countries and this variability is not explained by the known risks. This observation indicates that other factors might be involved in the initiation of PDAC. DISCERN, a project funded by the European commission has as goal to identify internal and external factors (exposome) associated with the initiation of PDAC among other cancers. In our work, we use induced pluripotent stem cells – iPSC- based models of pancreatic ductal organoids to experimentally validate the role of the identified exposome elements on the initiation of PDAC. Our investigation includes the high-throughput generation of pancreatic organoids from well-established stress/cancer-associated fluorescent reporter iPSC lines, combined with transcriptomic analysis both on the single-cell and population levels.   

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