Narrative cv coming soon 

Abstract: Osteosarcoma (OS) is the most common primary malignant bone tumour, yet its incidence remains low at 1-3 cases per million annually. Despite this rarity, patient prognoses remain poor, with limited therapeutic advances over the past five decades. IkB kinase alpha (IKKa) has emerged as a key driver of cancer progression, metastasis, and chemoresistance by promoting pro-inflammatory NF-kB signalling pathways and directly phosphorylating histone H3 at serine 10 on oncogene promoters. However, its role in OS remains unclear. This study aimed to identify IKKa-dependent genes in U2OS cells, a human OS cell line. Using transcriptomic analysis and further experimental validation, we investigated the effect of IKKa CRISPR-Cas9 knockdown on gene expression. The findings suggest a novel role for IKKa in regulating cholesterol biosynthetic enzymes, and subsequent cholesterol production. Furthermore, these findings provide compelling evidence for IKKa as a potential therapeutic drug target in cholesterol-related diseases.